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Design, Synthesis and Biological Evaluation of Triptorelin Analogs Containing Tetrazole Moiety
Author(s) -
Sohbati Hamidreza,
Alipour Mohsen,
Hosseinkhani Saman,
Balalaie Saeed,
Hamdan Fatima
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201903722
Subject(s) - tetrazole , moiety , chemistry , triptorelin , stereochemistry , docking (animal) , peptide , peptide bond , peptidomimetic , combinatorial chemistry , biochemistry , gonadotropin releasing hormone , medicine , nursing , hormone , luteinizing hormone
The design and synthesis of novel bioisosteric analogues of triptorelin acetate containing tetrazole moiety at C‐ or N‐terminus of the peptides is described. Tetrazole acetic acid was synthesized through the reaction of an alkyl cyanoacetate and sodium azide, after that, it was coupled to the N‐terminus of triptorelin instead of pyroglutamic acid. In another approach, tetrazole acetohydrazide was prepared and conjugated to the C‐terminus of triptorelin instead of the amide bond. The synthesized peptides containing tetrazole moiety at the C‐ or N‐terminus of the peptide sequence are peptidomimetics of triptorelin acetate. The docking results of the designed derivatives showed the same interaction of triptorelin with the receptor but with a higher score.The peptide that has tetrazole moiety in its C‐terminus, restricted the testosterone level during four weeks of in vivo study and led to keep testosterone at a moderate level during the first week compared to control.