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A Novel Fluorescent Probe for Detection of Hydrogen Sulfide and Its Bioimaging Applications in Living Cells
Author(s) -
Zhu Lei,
Liao Wenhao,
Chang Haizhen,
Liu Xianjun,
Miao Shaobin
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201903451
Subject(s) - fluorescence , lysosome , hydrogen sulfide , organelle , chemistry , fluorescence microscope , biophysics , rhodamine , fluorescence lifetime imaging microscopy , nanotechnology , biochemistry , biology , materials science , enzyme , physics , sulfur , organic chemistry , quantum mechanics
Hydrogen sulfide (H 2 S) is a critical gasotransmitter playing an important role in various physiological and pathological processes. The development of fluorescent probes for rapid, sensitive, and selective lysosomal H 2 S sensing has attracted much attention. Herein we report a rhodamine based probe MF‐N 3 that can selectively accumulate in lysosomes and give turn‐on fluorescence in the presence of both H 2 S and protons. Results show that MF‐N 3 has an exclusive response to H 2 S in the acidic lysosomal environment but does not respond in other organelles or in neutral environments. This strategy can circumvent the fluorescence interference from H 2 S of other sources, thus providing a high sensitivity and selectivity on lysosomal H 2 S imaging by fluorescence microscopy. This pH‐dependent lysosome‐targeting fluorescent probe has been successfully applied to lysosomal H 2 S imaging in living cells and is of great potential for applications to the environmental and biological systems.