Synthesis and Pharmacological Evaluation of Some Amide Functionalized 1 H ‐Benzo[ d ]imidazole‐2‐thiol Derivatives as Antimicrobial Agents

A series of novel amide functionalized 1 H ‐benzo[d]imidazole‐2‐thiol derivatives 4 a‐o were prepared from substituted benzimidazole‐2‐thiol 1 a‐c . All the final compounds were screened for antimicrobial, minimum bactericidal concentration (MBC) and anti‐biofilm activities against Gram‐positive and Gram‐negative bacterial strains. Amongst all the synthesized derivatives, compounds 4 c , 4 e , 4 f , 4 g , 4 k and 4 o exhibiting promising antibacterial activity against various bacterial strains were identified. Moreover, the compound 4 c showed highly significant antimicrobial activity (MIC value of 1.9 μg/mL) and broad‐spectrum anti‐biofilm activity which was identified as a potential lead molecule.