Premium
Design of an Immobilized Biohybrid Catalyst by Adsorption Interactions onto Magnetic Srebrodolskite Nanoparticles
Author(s) -
Morales Andrés H.,
Hero Johan S.,
Navarro María C.,
Farfán Elsa M.,
Martínez María A.,
Lamas Daniela L.,
Gómez María I.,
Romero Cintia M.
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201903306
Subject(s) - lipase , adsorption , chemistry , hydrolysis , thermal stability , biocatalysis , immobilized enzyme , catalysis , ionic strength , magnetic nanoparticles , chemical engineering , nanoparticle , thermal decomposition , ethanol , nuclear chemistry , organic chemistry , ionic liquid , enzyme , aqueous solution , engineering
In this work, a novel nanobiocatalyst (L–Mcn4@Ca 2 Fe 2 O 5 ) was synthesized from Bacillus lipase on srebrodolskite nanoparticles. Bacillus sp. MCn4 culture media was optimized reaching the maximum lipolytic activity using peptone 7.5 g/L and sucrose 5 g/L and Ca 2 Fe 2 O 5 , was synthesized and characterized (size of 100–150 nm). L–Mcn4@Ca 2 Fe 2 O 5 was obtained by adsorption (12 U/mg oxide) and successfully characterized by different techniques. According to the best immobilization conditions, pH 4 and 100 Mm ionic strength, an adsorption model with two stages was proposed: initial interfacial activation followed by multi‐point electrostatic interactions. Stability studies showed an enhancement in its performance between 50 and 70 °C and against ethanol compared with free enzyme and the thermal decomposition process was softer when lipase was immobilized. The biocatalyst hydrolyzed fish oil showing enrichment in the PUFAs content with an increase on DHA of 1.2 fold showing a great potential for its use in the food industry.