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2′‐5′‐Isomerically Linked Thrombin‐Binding Aptamer (isoTBA) Forms a Stable Unimolecular Parallel G‐Quadruplex in the Presence of Sr 2+ Ions
Author(s) -
Wagh Atish A.,
Fernandes Moneesha
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201902005
Subject(s) - g quadruplex , chemistry , antiparallel (mathematics) , phosphodiester bond , aptamer , ion , divalent , nuclease , stereochemistry , crystallography , dna , biochemistry , organic chemistry , rna , physics , genetics , quantum mechanics , biology , magnetic field , gene
The ability of the isomeri 2′‐5′‐phosphodiester‐linked thrombin‐binding DNA aptamer pentadecamer G 2 T 2 G 2 TGTG 2 T 2 G 2 (isoTBA), to fold into G‐quadruplex structures in the presence of different mono‐ and divalent cations is reported. Strikingly, Sr 2+ ions cause isoTBA to fold into a stable parallel unimolecular G‐quadruplex, in contrast to the antiparallel unimolecular G‐quadruplex fold observed in the presence of K + . IsoTBA being advantageously more stable than TBA to nuclease degradation, may thus be useful in the selective detection of Sr 2+ ions, which it can sense with a detection limit of ∼ 55 μM.