Microwave‐Assisted Synthesis and Biological Evaluation of Some New Pyrazolothiazoles via a Multicomponent Approach

A series of new pyrazolothiazole hybrids were designed and synthesized by the microwave ‐ assisted multicomponent reaction of thiosemicarbazide, 3‐(furan‐2‐yl)‐1‐(pyridin‐2‐yl)prop‐2‐en‐1‐one and 1‐(pyridin‐2‐yl)‐3‐(thiophen‐2‐yl)prop‐2‐en‐1‐one with substituted 2‐bromoacetophenones. Structures of all the synthesized compounds were in good agreement with their spectral (FTIR, 1 H & 13 C NMR, Mass) and analytical data (CHN analysis). The target compounds were screened for their in vitro cytotoxic activity by testing against human adenocarcinoma (A549), cervical cancer (HeLa), human neuroblastoma (SK−N‐SH) and human prostate cancer (DU‐145) cell lines. From the in vitro results, it was found that the compound derived from p ‐ NO 2 phenyl ( 4 h) has potent activity against HeLa cell line with IC 50 value 9.97 ± 0.05 μM. Also, the compounds having p ‐OCH 3 phenyl ( 4 c) , phenyl ( 4 i) , and p ‐Cl phenyl ( 4 j) substitutions exhibited significant activity against HeLa cell line with IC 50 values 19.78 ± 0.03 μM, 18.47 ± 0.01 μM and 15.61 ± 0.06 μM respectively.