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Ecofriendly Determinations of Pseudoephedrine HCl and Triprolidine HCl in Their Tablet Form: Evaluation of Dissolution Profile.
Author(s) -
Bassuoni Yasmine F.,
Essam Hebatallah M.,
Elzanfaly Eman S.,
Zaazaa Hala E.,
Kelani Khadiga O.
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201901222
Subject(s) - chromatography , pseudoephedrine , chemistry , derivative (finance) , high performance liquid chromatography , analytical chemistry (journal) , analyte , ephedrine , neuroscience , financial economics , economics , biology
Derivative ratio method was applied to determine PSE in the range of 5–1000 μg/mL by using 10 μg/mL TRI as a divisor, where the interference of TRI was cancelled at 265.90 nm. Second derivative method was applied to determine TRI in the range of 0.25–50 μg/mL, by selecting a zero crossing point at 304.80 nm. For the HPLC method, different experimental parameters were investigated and optimized to allow complete baseline separation and well resolved peaks. The analytes were partitioned between C 18 column (25 cm×4 mm) and phosphate buffer (pH 4.6): acetonitrile (60: 40, v/v) as a mobile phase with flow rate of 1 mL/min and UV detection at 254 nm. The HPLC method was valid in the range of 5–600 μg/mL for PSE and 0.2–50 μg/mL for TRI. The methods were validated in compliance with ICH guidelines, where acceptable results were obtained indicating the suggested methods validity.