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Sensitive Detection of Coralyne and Heparin Using a Singly Labeled Fluorescent Oligonucleotide Probe
Author(s) -
Zhu Xingxing,
Chen Miangjian,
Ma Changbei
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201900942
Subject(s) - fluorophore , fluorescence , chemistry , heparin , oligonucleotide , guanine , quenching (fluorescence) , oligomer restriction , photochemistry , biophysics , dna , biochemistry , biology , nucleotide , optics , physics , gene
Herein, we designed and developed a simple, rapid and sensitive method for detection of coralyne and heparin using a singly labeled fluorescent probe, based on photoinduced electron transfer (PIET). A specific oligonucleotide strand was labeled with fluorophore (6‐FAM) at its 5’ end and four guanines at its 3’ end. In the presence of coralyne, homo‐adenine DNA duplex is formed via A 2 ‐coralyne‐A 2 coordination, thus leading to the fluorescence quenching due to PIET between guanine and fluorophore. Upon the addition of heparin, the formation of heparin‐coralyne complex can prevent coralyne from forming A 2 ‐coralyne‐A 2 coordination, separating fluorophore from guanines; as a result, the fluorescence intensity can be recovered. The detection limits of the method with respect to coralyne and heparin were 3.1 nM and 0.04 nM (S/N=3), respectively. Moreover, the proposed method was successfully applied to detect heparin in human serum sample, further demonstrating that it can have promising practical applications.