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Preparation of a Poly(ethylene glycol)‐Based Cross‐Linked Network from a Click Reaction for Enzyme Immobilization
Author(s) -
Oktay Burcu,
Demir Serap,
KayamanApohan Nilhan
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201900296
Subject(s) - maleimide , covalent bond , polyethylene glycol , peg ratio , polymer chemistry , click chemistry , ethylene glycol , chemistry , immobilized enzyme , epoxy , linker , glutaraldehyde , yield (engineering) , organic chemistry , enzyme , materials science , computer science , finance , economics , metallurgy , operating system
In this study, a polyethylene glycol (PEG)‐maleimide film was prepared by a thiol‐ene click reaction. The covalent immobilization of amylase was carried out onto the PEG‐maleimide based photo‐curable network via epoxy and C=O groups without any linker. The epoxy and carbonyl groups on the PEG backbone improved the affinity of the enzyme. The immobilization yield of amylase onto the film was 72.46%. The immobilization capacity was also determined as 313 mg/g. The thermal stability of amylase improved via covalent attachment. Immobilized amylase showed maximum activity at 40 °C and pH 6.5. The immobilized amylase continued to retain 66% of its initial activity after 30 days.
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