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Synthesis of Gold Nanoparticles Using Citrus macroptera Fruit Extract: Anti‐Biofilm and Anticancer Activity
Author(s) -
Majumdar Moumita,
Biswas Suresh Chandra,
Choudhury Rupasree,
Upadhyay Priyanka,
Adhikary Arghya,
Roy Dijendra Nath,
Misra Tarun Kumar
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201804021
Subject(s) - colloidal gold , biofilm , surface plasmon resonance , bacteria , chemistry , nanoparticle , biophysics , nuclear chemistry , pseudomonas aeruginosa , bacterial cell structure , cancer cell , materials science , nanotechnology , cancer , biology , genetics
Citrus macroptera (CM) fruit juice was used to reduce gold ions (Au 3+ ) and to stabilize as‐formed gold nanoparticles (AuNPs). CM‐AuNPs exhibit surface Plasmon resonance (SPR) peak at 544 nm. Transmission Electron Microscope (TEM) study reveals that they are multi‐shaped, predominantly pseudo‐spherical with a diameter 20 nm. They have surface charge −30 mV and possess FCC lattice pattern. Particles could effectively inhibit the biofilm formation of Pseudomonas aeruginosa bacteria. Experimental study shows that CM‐AuNPs make the bacteria cripple to generate EPS and pyocyanin. AFM images of the structure of biofilm reveal that CM‐AuNPs indeed efficiently disrupt the biofilm structure. CM‐AuNPs in combination with Sub‐MIC dose of gentamicin against P. aeruginosa bacteria exhibit quite high efficiency. The cytotoxic effect of CM‐AuNPs against three human cancer cell line show that they are comparatively more efficient to regulate the growth of HepG2 (liver cancer cell line) than that of A 549 (adenocarcinogenic human alveolar basal epithial cell) and MDA‐MB 468 (breast cancer cell). Thus, the CM‐AuNPs could be treated as a potential anti‐biofilm and anti‐cancer agent.