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Pt(II)‐Based Artificial Nitroreductase: An Efficient and Highly Stable Nanozyme
Author(s) -
Aghahosseini Hamideh,
Tabatabaei Rezaei Seyed Jamal,
Maleki Mahshid,
Abdolahnjadian Davod,
Ramazani Ali,
Shahroosvand Hashem
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201803776
Subject(s) - nitroreductase , platinum , chemistry , catalysis , rational design , combinatorial chemistry , ligand (biochemistry) , substrate (aquarium) , active site , aqueous solution , enzyme , stereochemistry , nanotechnology , materials science , biochemistry , organic chemistry , biology , ecology , receptor
The nitroreductases are a family of natural enzymes with great importance. Inspired the integration of biology and nanotechnology, herein, a rational design for the preparation of the platinum(II)‐based artificial nitroreductase is reported. Engineering the individual ligand scaffold, which on the one hand could form a highly stable complex with the platinum(II) as the catalytic active site and on the other hand could provide halogen bonding site for induce catalysis by approximation, creates an efficient and long‐term resistance artificial nitroreductase. The platinum(II)‐based artificial nitroreductase was characterized by FTIR, TEM, XRD, EDAX and ICP techniques. The reaction conditions were fully optimized for the reduction of high concentration p ‐nitrophenol as a specific substrate in aqueous solution employing experimental design, which highlights the potential application of the designed artificial nitroreductase in wastewater treatment. Moreover, the enzyme‐like Michaelis‐Menten kinetics of the reaction was investigated. The prepared platinum(II)‐based artificial nitroreductase could be stable even after 20 catalytic cycles with excellent activity and it was represented a good turnover frequency.