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Spectrofluorometrical Determination of Vitamin B 1 in Different Matrices Using Box‐Behnken Designed Pipette Tip Solid Phase Extraction by a Carbon Nanotube Sorbent
Author(s) -
Hashemi Sayyed Hossein,
Yahyavi Hossein,
Kaykhaii Massoud,
Hashemi Mohammad,
Mirmoghaddam Majid,
Keikha Ahmad Jamali
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201803731
Subject(s) - box–behnken design , sorbent , chromatography , extraction (chemistry) , elution , detection limit , calibration curve , materials science , ionic strength , carbon nanotube , response surface methodology , solid phase extraction , analytical chemistry (journal) , chemistry , nanotechnology , adsorption , aqueous solution , organic chemistry
This study describes development of a method for spectrofluorometrical determination of thiamine (vitamin B 1 ) in vitamin tablets, cactus fruits, multivitamin syrups, and human plasma after its extraction by pipette tip micro‐solid phase extraction using carbon nanotube as sorbent. A response surface methodology was used for experimental design to obtain optimal response. Ten variables including pH of the sample solution, type and volume of eluent solvent, volume of sample solution, effect of ionic strength, concentration of K 3 [Fe(CN) 6 ] (as derivatizing agent), concentration of NaOH, number of cycles of extraction and elution and amount of sorbent were regarded as factors in the optimization study by Box‐Behnken design. Figures of merit were calculated as: detection limit; 0.16 μg L −1 , enrichment factor; 91, linear calibration curve in the range of 0.5‐130.0 μg L −1 ; and reproducibility (as RSD %) better than 2.4%. The method was applied to the determination of different real samples successfully.