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Sensitive Colorimetric Assay Based on Peroxidase‐Like Activity of CeO 2 Nanoparticles Supported on SBA‐15 Mesoporous Silica to Determination of H 2 O 2
Author(s) -
Oliveira Larissa H.,
de Barros Anerise,
Pinto Lidiane O.,
Oliveira Cristine S.,
Kubota Lauro T.,
Sigoli Fernando A.,
Mazali Italo O.
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201803709
Subject(s) - thermogravimetric analysis , mesoporous silica , substrate (aquarium) , nanoparticle , catalysis , nanomaterials , mesoporous material , raman spectroscopy , materials science , nuclear chemistry , peroxidase , transmission electron microscopy , chemical engineering , small angle x ray scattering , spectroscopy , high resolution transmission electron microscopy , analytical chemistry (journal) , chemistry , nanotechnology , chromatography , scattering , organic chemistry , enzyme , optics , oceanography , physics , engineering , quantum mechanics , geology
Abstract We report a novel combination of nanomaterial proposed as a peroxidase‐like enzymatic mimic for detection of H 2 O 2 , where CeO 2 nanoparticles dispersed on SBA‐15 through synthesis by the impregnation‐decomposition cycles method (IDC). The system displays a good peroxidase‐like activity and catalyzes to the oxidation of 3,3',5,5‘‐tetramethylbenzidine (TMB) by H 2 O 2 . K M values were calculated between 0.04 and 0.08 mM for different (CeO 2 /SBA‐15) x catalyst system, the results demonstrates that the combination of these materials displayed a good affinity to TMB substrate, consequently improving the colorimetric assay, due to uniform mesostructures of SBA‐15 and their high surface areas. A detailed study of synergistic effects between CeO 2 NPs and SBA‐15 support were held in order to understand how these effects can improve the colorimetric assay. Additionally, high resolution transmission electron microscopy (HR‐TEM), energy dispersive X‐ray spectroscopy (EDX), Raman spectroscopy, thermogravimetric analysis (TGA), small angle X‐ray scattering (SAXS) and textural properties were utilized for characterization of the prepared catalysts systems.