Premium
A Pyrene‐Based Turn‐On Fluorescence Probe for CN − Detection and Its Bioimaging Applications
Author(s) -
Chao Jianbin,
Xu Miao,
Liu Yaoming,
Zhang Yongbin,
Huo Fangjun,
Yin Caixia,
Wang Xiaolu
Publication year - 2019
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201803578
Subject(s) - fluorescence , detection limit , chemistry , pyrene , cyanide , analytical chemistry (journal) , selectivity , turn (biochemistry) , photochemistry , linear range , inorganic chemistry , chromatography , catalysis , optics , organic chemistry , physics , biochemistry
The fluorescent probe 1‐(pyren‐1‐yl)‐3‐(2, 6‐dichlorophenyl)‐acrylketone (probe 1) was synthesized for the detection of cyanide (CN − ). The results indicated that CN − significantly increased the fluorescence intensity of probe 1 at 464 nm. The fluorescence intensity showed a good linear relationship with the concentration of CN − in the range of 2.5‐12.5 μM, and the detection limit was 37 nM. Probe 1 exhibited outstanding selectivity toward CN − . Reaction mechanism of CN − nucleophilic addition to probe 1 was supported by DFT and TD‐DFT calculations. In addition, probe 1 had been successfully employed for the imaging of CN − in cells, C. elegans and zebrafishes. Besides, the probe 1 was also successfully applied to detect the concentration of CN − in spiked tap water samples.