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Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One‐Use ‘Sensing in‐a‐Drop’ Applications
Author(s) -
Sanli Serdar,
Celik Emine Guler,
Demir Bilal,
Gumus Z. Pinar,
Ilktac Raif,
Aksuner Nur,
Demirkol Dilek Odaci,
Timur Suna
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201802602
Subject(s) - biomolecule , biosensor , glucose oxidase , materials science , nanofiber , surface modification , magnetic nanoparticles , analyte , dielectric spectroscopy , nanoparticle , chemical engineering , nanotechnology , polymer , x ray photoelectron spectroscopy , electrochemistry , electrode , chemistry , composite material , chromatography , engineering
Stabilization of biomolecules on matrices is critically important. Here we constructed composite nanofibers with magnetic features by electro‐spinning of magnetic nanoparticles (MNP) in an appropriate polymer matrix on a collector surface for multiple uses. ‘Poly(vinylalcohol)‐polyethyleneimine/Fe 3 O 4 magnetic nanoparticles’ (PVA‐PEI/MNP) composite nanofiber was used on the screen printed electrodes (SPE) to serve as a platform for the glucose oxidase (GOx) biofilm as the model biomolecule. To show the potential application of this material as a biosensor component, the resulted biofilm, called ‘PVA‐PEI/MNP/GOx’, was fixed on SPE via a neodymium magnet for the electrochemical detection of glucose at −0.7 V where oxygen consumption due to enzymatic reaction was measured. The main advantage of the obtained magnetic biomembrane is that it allows analysis with a single drop. After step‐by‐step surface modifications, analytical characterization was performed using various techniques, such as voltammetry, electrochemical impedance spectroscopy, Fourier‐transform infrared spectroscopy and X‐Ray photoelectron spectroscopy as well as microscopic techniques. The linear range of the PVA‐PEI/MNP/GOx surface was found as 0.0125 to 0.5 mM with a limit of detection of 11.5 μM for glucose as analyte. The application of PVA‐PEI/MNP/GOx for glucose detection in synthetic samples was carried out. Data proved the potential use of the ‘PVA‐PEI/MNP’ surface as a biomolecule immobilization platform for disposable biosensor applications.