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A Simple Perceptive Diphenyl‐Imidazole‐Based Dipodal Schiff‐Base Chemosensor for Zn 2+ and PPi ions and Its Live‐Cell Imaging Applications
Author(s) -
Gomathi Asaithambi,
Vasanthi Mani,
Viswanthamurthi Periasamy,
Suresh Shanmugan,
Nandhakumar Raju
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201802233
Subject(s) - chemistry , fluorescence , imidazole , schiff base , ion , detection limit , naked eye , binding constant , selectivity , pyrophosphate , zinc , nuclear chemistry , analytical chemistry (journal) , stereochemistry , binding site , chromatography , catalysis , organic chemistry , biochemistry , physics , quantum mechanics , enzyme
2,6‐bis(( E )‐(2‐(benzo[d]thiazol‐2‐yl)hydrazono)methyl)‐4‐(4,5‐diphenyl‐1H‐imidazol‐2‐yl)phenol (PIS) conjugate has been designed as a selective fluorescent probe for zinc ion. The PIS exhibits selectivity towards Zn 2+ among various ions in DMSO‐H 2 O (1:9) v/v 50 mM HEPES (4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid) at pH=7.4. Moreover, the detection of PIS towards Zn 2+ ion could be realized in “ naked eye” with a color conversion from yellow to greenish yellow. The linear relationship between fluorescence intensity and Zn 2+ ion concentration indicates that PIS can be used for quantification of Zn 2+ ion. The PIS probe can be used in broad pH ranging from 4 to 9. The limit of detection (LOD) was found to be 0.52 nM for Zn 2+ ion. The binding ratio and binding constant of PIS‐Zn 2+ complex were determined and calculated as 1:1 and 2.36×10 6 M −1 according to Job′s plot and Benesi–Hildebrand equation, respectively. Furthermore, the PIS‐Zn 2+ complex subsequently acted as a secondary fluorescent turn‐off probe for pyrophosphate (PPi) ion recognition among the other anions. To assess the real‐world efficacy of the PIS and PIS‐Zn 2+ complex, they were evaluated by test paper strips. Likewise, the probe PIS and PIS‐Zn 2+ complex were used as in vitro cell imaging probe, and successfully demonstrated as feasible bio‐marker as well.