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pH‐Driven Precise Control of Hybridization Reaction Kinetics for Rapid DNA Assay
Author(s) -
Yan Yishu,
Lu Yuan,
Liu Xiaoni,
Zhang Yan,
Chen Jinghua
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201801786
Subject(s) - colloidal gold , dna , dna–dna hybridization , combinatorial chemistry , kinetics , chemistry , nanotechnology , robustness (evolution) , nucleic acid thermodynamics , aqueous solution , hybridization probe , nanoparticle , materials science , biological system , biochemistry , biology , base sequence , organic chemistry , gene , physics , quantum mechanics
The development of DNA assays based on gold nanoparticles (AuNPs) holds great promise for point‐of‐care diagnosis at molecular level; nevertheless, how to accelerate the hybridization reaction rates, and design rapid DNA assay in aqueous solution remains challenging. Herein, a pH switchable AuNP aggregation system, functionalized with compound Y1, was set up. This method provided an elegant strategy for the precise control of DNA hybridization reaction kinetics when Y1 was co‐functionalized with ssDNA on the AuNPs. Using this strategy, real‐time DNA detection was realized by simply adjusting the pH value of the solution without affecting the sensitivity. Particularly worth mentioning, this system maintained high specificity, and were capable of accurately discriminating against single‐based mismatched sequences. In total, this system significantly improved the rapidity and robustness of ssDNA modified AuNPs, providing sound basis for the low‐cost, and disposable molecular diagnosis design.