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His‐Tag‐Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples
Author(s) -
Turan Eylem
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201801557
Subject(s) - epitope , chemistry , horseradish peroxidase , molecular imprinting , molecularly imprinted polymer , methacrylate , antigen , imprinting (psychology) , combinatorial chemistry , chromatography , monomer , polymer , biochemistry , selectivity , organic chemistry , enzyme , catalysis , biology , genetics , gene
In the epitope imprinting method, a short peptide, even without a defined tertiary structure, can be used as the template to produce protein‐selective polymers. In this study, His‐tag‐epitope imprinted magnetic nanoparticles (EMIP‐MNPs) were prepared via epitope imprinting method onto the surface of the magnetic nanoparticles for specific recognition and separation of thyroid peroxidase antigens (TPO). 3‐(Trimethoxysilyl) propyl methacrylate (TMSPM), copper (II) acrylate (Cu(II)Ac) and N ‐isopropylacrylamide (NIPAAm) were chosen as functional monomers. Ethyleneglycoldimethacrylate (EGDMA) and 2,2’‐azobisisobutyronitrile (AIBN) were selected as crosslinking agent and initiator, respectively. The EMIP‐MNPs were prepared within 30 min by epitope imprinting method which was shorter than the surface imprinting method. The prepared EMIP‐MNPs were thermosensitive and showed selective affinity to target TPO antigens. Moreover, the EMIP‐MNPs were easily and quickly recognized and separated to target antigens from the whole blood sample.