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Colloidal Stability of Lipid/Protein‐Coated Nanomaterials in Salt and Sucrose Solutions
Author(s) -
Nobeyama Tomohiro,
Mori Megumi,
Shigyou Kazuki,
Takata Koji,
Pandian Ganesh N.,
Sugiyama Hiroshi,
Murakami Tatsuya
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201801180
Subject(s) - nanorod , nanomaterials , zeta potential , chemistry , colloid , cationic polymerization , salt (chemistry) , aqueous solution , sucrose , chemical engineering , lipid bilayer , membrane , biophysics , nanoparticle , biochemistry , organic chemistry , engineering , biology
Considering the importance of surface chemistry in the colloidal stabilization of gold nanorods (AuNRs), we designed AuNRs in two physiologically relevant, salt and salt‐free aqueous solutions to validate its role in colloidal stability. Here, we show that AuNRs coated with cationic lipid/lipid‐binding protein composite materials is stable in both phosphate‐buffered saline (PBS) and sucrose solution (200 mM), but not in deionized water. Salting‐in effects of proteins explain the relatively better stability of AuNRs in PBS than in deionized water. Sucrose‐assisted stabilization of the AuNRs was associated with a decrease in their zeta potential and tended to diminish without the cationic lipid, which would be attributed to an electrostatic interaction between sucrose and the cationic lipid. Given that sucrose solution is often used for cell‐sized liposome studies, our finding will enable nanomaterial‐membrane interaction analyses in live cell and cell‐free systems with identical nanomaterials.