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Long Chain Effects on DNA‐Binding and Cytotoxicity to Cancer Cells in Metallo‐Supramolecular Oligomers
Author(s) -
Rana Utpal,
Hossain Md. Delwar,
Chakraborty Chanchal,
Nagano Reiko,
Morita Hiromi,
Hattori Shinya,
Minowa Takashi,
Higuchi Masayoshi
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201800961
Subject(s) - cytotoxicity , annexin , chemistry , apoptosis , flow cytometry , dna , monomer , binding constant , supramolecular chemistry , pi , polymerization , dimer , staining , molar ratio , microbiology and biotechnology , polymer chemistry , biochemistry , in vitro , binding site , organic chemistry , molecule , biology , polymer , genetics , catalysis
We synthesized Fe(II)‐based metallo‐supramolecular oligomers with the different degree of polymerization (DP) (DP‐1, −5 and −10) by changing the molar ratio of Fe(II) acetate and bis(terpyridyl)benzene to 1:2, 5:6, and 10:11, respectively. We investigated the chain length effects on the binding affinity to DNA and cytotoxicity to cancer cells in the oligomers. DP‐10 showed five‐time larger DNA binding constant and four‐time stronger cytotoxicity than DP‐1. We also studied the cell apoptosis assay using annexin V‐FITC/PI staining by flow cytometry and revealed that the ratio of early and late apoptosis cells increased from 15.6% (DP‐1) to 61.4% (DP‐10) with increasing the chain length.