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A Multifunctional Biomimicking Oxidovanadium(V) Complex: Synthesis, DFT Calculations, Bromo‐peroxidation and DNA Nuclease Activities
Author(s) -
Palmajumder Eshita,
Dash Soumya Ranjan,
Mitra Joyee,
Mukherjea Kalyan K.
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201800817
Subject(s) - chemistry , dna , nuclease , puc19 , cleavage (geology) , stereochemistry , phosphodiester bond , fluorescence , crystallography , plasmid , biochemistry , rna , physics , geotechnical engineering , quantum mechanics , fracture (geology) , engineering , gene
An oxidovanadium(V) complex [VO(SGly)(bpy)]ClO 4 (H 2 SGly= N ‐(2‐hydroxybenzyl)‐L‐glycine; bpy=2,2′‐bipyridine) was synthesized and characterized by spectroscopic studies (IR, UV‐vis, ESI‐MS). The theoretical structure of the complex was optimized by density functional theory (DFT) calculations. The multifunctionality of this oxidovanadium complex was demonstrated by the application of it as a catalyst in peroxidative bromination of single‐pot conversion of phenol red to bromophenol blue, whereas its nuclease activity was established by DNA cleavage. The interaction of this oxidovanadium complex with DNA was studied by various physicochemical tools like UV‐vis, fluorescence, and viscosity measurement studies. The intrinsic binding constant of the complex with DNA was determined spectrophotometrically and calculated to be (2.3 ± 0.02) x 10 4 M −1 . The spectroscopic studies and the viscosity measurements indicate that the complex binds to the groove of CT‐DNA, but whether to the major or minor groove that was not established. The extent of vanadium complex induced DNA cleavage was studied by gel electrophoresis techniques. The complex was found to promote cleavage of pUC19 plasmid DNA from the super coiled (SC DNA) form I to nicked coiled (NC DNA) relaxed form II.