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PCR under Low Ionic Concentration Buffer Conditions
Author(s) -
Takei Fumie,
Akiyama Misaki,
Nobusawa Kazuyuki,
Sabani Norhayati Binti,
Han Huanwen,
Nakatani Kazuhiko,
Yamashita Ichiro
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201702542
Subject(s) - tris , polymerase chain reaction , buffer (optical fiber) , chemistry , chromatography , buffer solution , ionic bonding , microbiology and biotechnology , polymerase , dna , real time polymerase chain reaction , ion , biology , biochemistry , gene , telecommunications , organic chemistry , computer science
A new polymerase chain reaction (PCR) system using a low‐salt concentration buffer was developed, which is suitable for electrical PCR process monitoring. Using Taq polymerase in 10 mM of Tris‐HCl buffer without KCl, the PCR progressed and the target DNA PCR products were produced. As the ionic concentration in PCR buffer solution decreased, the quantity of PCR products decreased, but the PCR products could be detected down to a total ion concentration of 5 mM. Single nucleotide polymorphism typing was also successful under 10 mM of Tris‐HCl without KCl. Both alleles (G or T) were detected clearly.