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Development and In vitro Anticancer Evaluation of Self‐Assembled Supramolecular pH Responsive Hydrogels of Carboxymethyl Chitosan and Polyoxometalate
Author(s) -
AlRashida Mariya,
Haider Ali,
Kortz Ulrich,
Joshi Sachin A.,
Iqbal Jamshed
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201702253
Subject(s) - self healing hydrogels , thermogravimetric analysis , differential scanning calorimetry , chitosan , polymer chemistry , nuclear chemistry , chemistry , swelling , fourier transform infrared spectroscopy , kinetics , materials science , chemical engineering , organic chemistry , physics , engineering , composite material , thermodynamics , quantum mechanics
Self‐assembled supramolecular pH‐responsive hydrogels of carboxymethyl chitosan (CMCh) and the trilacunary heteropolytungstate salt Na 12 [α‐P 2 W 15 O 56 ].24H 2 O generating the free polyanion P 2 W 15 in solution, have been synthesized via electrostatic interactions. Benzoyl peroxide (BPO) was used as initiator for grafting poly (methacrylic acid) on polymer skeleton. The developed transparent hydrogels were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X‐ray powder diffraction (XRD) and thermal analysis including thermogravimetric analyses and differential scanning calorimetry (TGA/DSC) and the results confirmed the successful hydrogel formation. The dynamic swelling index and the controlled release pattern of the prepared hydrogels were evaluated at pH 1.2 and 7.4. The maximum swelling index and polyanion release were observed at pH 7.4. Different mathematical models including first order, zero order, Korsmeyer‐Peppas model and Higuchi model were applied and suggested that the release of encapsulated P 2 W 15 follows the super case transport‐II mechanism and zero order kinetics. An evaluation of the anticancer activity and cytotoxicity profiling showed that both encapsulated P 2 W 15 (hydrogel) and free P 2 W 15 possessed a good, dose‐dependent anticancer activity against MCF‐7 and Hela cells, with only minimal cytotoxic effects on normal cells (Vero).