Premium
Development and Application of an NMR‐Based Assay for Polyphenol Oxidases
Author(s) -
Li Yu,
Zafar Ayesha,
Kilmartin Paul A.,
Reynisson Jóhannes,
Leung Ivanhoe K. H.
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201702144
Subject(s) - polyphenol , polyphenol oxidase , chemistry , virtual screening , enzyme , biochemistry , tandem , combinatorial chemistry , antioxidant , drug discovery , materials science , peroxidase , composite material
Polyphenol oxidases (PPOs) are enzymes that catalyse the oxidation of phenolic compounds. We report a NMR‐based assay that can be used as a screening and validation tool for PPO activity modulators, which was demonstrated using a series of PPO inhibitors and antioxidants. This method is exemplified by work in tandem with virtual screening and molecular modelling, which led to the discovery of several novel PPO inhibitors.