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Merocyanine Dye‐Based Fluorescent Chemosensor for Highly Selective and Sensitive Detection of Hypochlorous Acid and Imaging in Live Cells
Author(s) -
Ponnuvel Kandasamy,
Ramamoorthy Jaganathan,
Sivaraman Gandhi,
Padmini Vediappen
Publication year - 2018
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201701833
Subject(s) - hypochlorous acid , merocyanine , fluorescence , photochemistry , chemistry , naked eye , detection limit , indole test , titration , iodide , proton nmr , organic chemistry , photochromism , chromatography , physics , quantum mechanics
A merocyanine dye‐based fluorescent probe have been synthesised for selective and sensitive detection of hypochlorous acid. The merocyanine dye 1 can be synthesized by simple condensation reaction of 1, 1, 2, 3‐tetramethyl‐1H‐benzo[e]indol‐3‐ium iodide with indole‐3‐carboxaldehyde. The reaction between hypochlorous acid and dye 1 is linear in the hypochlorous acid concentration range of 0 to 100 μM with a detection limit of 42 nM by fluorescent spectrometry method. The intramolecular charge transfer based fluorescent probe dye 1 displayed a considerable fluorescent spectrum at 425 nm enhancement and the color changes from red to colorless by naked eye detection in the addition of hypochlorous acid. Further supported by the 1 H NMR titrations reveal that the reactive oxygen species in dye 1 bind to the indole‐NH‐unit. The chemosensor also showed excellent performance when used in the “test strip” method.