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Target‐Regulated Ce 3+ /Ce 4+ Redox Switch for Fluorescence Turn‐on Detection of H 2 O 2 and Glucose
Author(s) -
Jiang Chao,
Zhang Yu,
Shen Haixia,
Liu Chenghui
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201701751
Subject(s) - calcein , glucose oxidase , redox , fluorescence , detection limit , chemistry , biosensor , photochemistry , analytical chemistry (journal) , inorganic chemistry , chromatography , biochemistry , membrane , physics , quantum mechanics
A facile fluorescence turn‐on assay for the sensitive detection of H 2 O 2 and glucose is developed based on the target‐regulated redox state switch between Ce 3+ and Ce 4+ . In this study, Ce 3+ can efficiently quench the fluorescence of calcein while Ce 4+ will not. Therefore, in the fluorescence‐quenched Ce 3+ /calcein‐based system, the H 2 O 2 ‐catalyzed oxidation of Ce 3+ to Ce 4+ will lead to the fluorescence recovery of calcein, which can be recorded for the quantitation of H 2 O 2 . More interestingly, CeO 2 nanoparticle, a fascinating material, exposes both Ce 3+ and Ce 4+ in a redox equilibration state on its surface. The H 2 O 2 ‐regulated Ce 3+ / Ce 4+ redox state switch on CeO 2 surface will be much easier and faster than that in the solution. Therefore, a CeO 2 /calcein complex‐based sensing system is further developed, and the detection limit of H 2 O 2 can be pushed down to a low concentration of 8 nM, which is nearly three orders of magnitude lower than that of the Ce 3+ /calcein‐based sensing mechanism. Furthermore, by using the CeO 2 /calcein‐based system, glucose can be also sensitively detected with the assistance of glucose oxidase (GOx), and as low as 200 nM glucose can be clearly discriminated from the blank control. This proposed strategy provides a simple, cost‐effective, and highly sensitive approach for detecting H 2 O 2 , glucose as well as other oxidases‐related bioreactions, which may serve as a powerful tool for biosensing and clinical diagnostics.