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Controlled‐Release‐Based Ultrasensitive and Highly Selective Turn‐On Fluorescent Mercury Biosensor
Author(s) -
Wang Wei,
Li Xin,
Hou Xiaoshan,
Wang Shiying,
Wang Fengying,
Luo Xiliang
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201701704
Subject(s) - biosensor , fluorescence , analyte , chemistry , detection limit , molecule , nanocages , small molecule , molecular recognition , nanotechnology , molecular probe , combinatorial chemistry , biophysics , dna , materials science , chromatography , biochemistry , organic chemistry , biology , physics , quantum mechanics , catalysis
A novel fluorescent biosensor for ultrasensitive and highly selective detection of Hg 2+ was developed by coupling Hg 2+ ‐induced conformational change of single‐stranded DNA recognition probe with T‐rich sequence and controlled‐release of cargo molecules loaded by Au nanocages with T–Hg 2+ –T coordination chemistry. A detection limit of as low as 1.4×10 −12 M with an excellent selectivity toward Hg 2+ could be achieved. The developed amplification strategy based on the controlled‐release of fluorescent molecules reached an extraordinary sensitivity of analyses, and may be further extended for the detection of a large class of analytes including protein, DNA and biological small molecules by combining molecular gate technology. The results of fluorescence microscopy imaging in living cells indicate that the fabricated system is an efficient controlled‐release biosensor in response to intracellular target molecules and predict its potential use for in situ molecular imaging in living systems.