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Lignin Exhibits Recalcitrance‐Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants
Author(s) -
Akinosho Hannah,
Yee Kelsey,
Rodriguez Miguel,
Muchero Wellington,
Yoo Chang Geun,
Li Mi,
Thompson Olivia,
Pu Yunqiao,
Brown Steven,
Mielenz Johnathan,
Ragauskas Arthur J.
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201701572
Subject(s) - clostridium thermocellum , lignin , lignocellulosic biomass , cellulosic ethanol , chemistry , hemicellulose , cellulose , bioprocess , enzymatic hydrolysis , biomass (ecology) , depolymerization , biochemistry , food science , cellulase , enzyme , organic chemistry , biology , agronomy , paleontology
Because cellulosic ethanol production remains cost‐prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash ethanol production costs through process integration. Clostridium thermocellum , a CBP microorganism, organizes its enzymes in a multi‐enzyme complex ‐ a stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit the extent of biomass deconstruction. Herein, six Populus were treated with C. thermocellum (ATCC 27405) and characterized to determine structural changes that resulted from CBP. The 2D HSQC NMR spectra of lignin‐enriched residues revealed that higher S/G ratio (2.6) and fewer carbon‐carbon interunit linkages (generally 2–5%) were present in the top performing poplar. Furthermore, cellulose degree of polymerization data suggests that C. thermocellum likely circumvents long chain cellulose, while cellulose crystallinity and hemicellulose molecular weight data do not provide a direct indication of features connected to recalcitrance. Hence, C. thermocellum is similarly impacted by the proposed lignin properties that negatively impact biomass deconstruction using fungal enzymes.