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D‐ a TNA Circuit Orthogonal to DNA Can Be Operated by RNA Input via SNA
Author(s) -
Murayama Keiji,
Nagao Ryuya,
Asanuma Hiroyuki
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201701126
Subject(s) - signal (programming language) , dna , nucleic acid , rna , electronic circuit , seesaw molecular geometry , chemistry , biology , computer science , engineering , biochemistry , physics , electrical engineering , gene , neutrino , nuclear physics , programming language
For a signal amplification system that is orthogonal to DNA, we designed a simplified seesaw gate composed of only D‐ a TNA. This new system performed signal amplification by toehold exchange reaction just as the DNA circuit did. Moreover, the D‐ a TNA circuit was not affected by natural nucleic acids carrying sequences complementary to the D‐ a TNA. In the presence of an SNA interface, however, an RNA signal was converted to D‐ a TNA signal, resulting in successful activation of D‐ a TNA circuit. This system can be used to design signal‐amplification circuits that are not influenced by contaminating DNA and RNA.