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A Label‐Free Fluorescent Sensor Based on Structure‐Switching Oligonucleotides for the Detection of Ag + , Biothiols and Acetylcholinesterase Activity
Author(s) -
Zhang Baozhu,
Wei Chunying
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201701089
Subject(s) - acetylcholinesterase , detection limit , cysteine , fluorescence , oligonucleotide , aché , chemistry , biophysics , enzyme , combinatorial chemistry , biochemistry , chromatography , biology , physics , optics , dna
A label‐free, highly sensitive and specific fluorescent sensor was developed for the detection of Ag + , biothiols and acetylcholinesterase (AChE) activity and for the screening of AChE inhibitors. The sensing approach was based on the conformational switch of human telomeric G‐rich sequence. The present assay was capable of detecting Ag + in the range of 0 to 6.0 μM, with a detection limit of 34 nM. In addition, the proposed method was developed as a turn‐on cysteine‐sensing system, which presented a good response to cysteine in the range of 0 to 4.5 μM, and the detection limit was 25 nM. The cysteine‐sensing system could be used to detect the activity of AChE as low as 0.05 U/L in the range of 0.05 to 1.3 U/L. The proposed sensor was also promising in screening of AChE inhibitors. Furthermore, this method has been applied successfully to the sensing of Ag + in tap water and lake water with satisfactory recovery.