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Molecular Interaction Kinetics and Mechanism Study of Phytohormones and Plant Protein with Fluorescence and Synchronous Fluorescence Techniques
Author(s) -
Zheng YuanYuan,
Sun Ning,
Xu MiaoHan,
Lu YuJing,
Qiu Bin,
Cheng MingJun,
Wong WingLeung,
Chow CheukFai
Publication year - 2017
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201700402
Subject(s) - quenching (fluorescence) , kinetics , chemistry , fluorescence , biophysics , plant growth , soy protein , adduct , protein–protein interaction , mechanism of action , biochemistry , biology , organic chemistry , botany , in vitro , physics , quantum mechanics
Cytokinins (CTKs) are widely used in agriculture to enhance crop production. To understand the role of CTKs in plant growth is vital, however, molecular mechanisms underlying CTKs action particularly their binding kinetics with proteins are not clear. The study of these interactions can obtain important information on how these signal molecules regulate cellular processes in targeted cells locally or transport to other functional parts of plants. In this study, the interactions between two CTKs and soy protein were investigated. The binding constants determined at 30 o C were K KT‐soy = 1.5×10 3 M −1 and K IP‐soy =0.67×10 2 M −1 and the binding stoichiometry was n KT‐soy =0.530 and n IP‐soy =0.5165 respectively. Moreover, the CTK‐protein interaction is found to be a static quenching process via an energy transfer mechanism. The adduct formed through electrostatic interactions under the plant acidity conditions was thus transported to the action parts of the plant to control growth process.