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Counterintuitive Solid‐State Syntheses of Indium‐Thiolate‐Phen Cations as Efficient and Selective Fluorescent Biosensors for HIV‐1 ds‐DNA and Sudan Ebolavirus RNA Sequences
Author(s) -
Li FuLing,
Yang ShuiPing,
Zhang WenHua,
Liu Quan,
Yu Hong,
Chen JinXiang,
Lang JianPing
Publication year - 2016
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201600554
Subject(s) - chemistry , dna , fluorescence , stacking , phenanthroline , fluorophore , oligonucleotide , stereochemistry , stoichiometry , hydrogen bond , crystallography , molecule , biochemistry , organic chemistry , physics , quantum mechanics
Abstract Coordination compounds [In(Tab) 2 (Phen) 2 ](PF 6 ) 3 ( 2 ), [In(Tab) 2 (Phen) 2 ⋅In(Tab) 4 (Phen)](OAc) 6 ( 3 ) and [In(Tab) 4 (Phen)](OAc) 3 ( 4 ) (Tab=4‐(Trimethylammonio)benzenethiolate; Phen=1,10‐Phenanthroline) are formed via a counterintuitive solid‐state synthesis involving the mix of In(OAc) 3 , TabHPF 6 and Phen in various stoichiometries. The Tab‐to‐Phen ratios of 2 − 4 are opposite to the TabHPF 6 ‐to‐Phen ratios of the starting materials. By comparison, the corresponding solution‐state reactions provide 4 exclusively at high concentrations, and otherwise the Phen‐free [In(Tab) 4 ](OAc) 3 ( 1 ). Reaction of In(OAc) 3 , TabHPF 6 and a bulkier Dpphen (Dpphen=4,7‐Diphenyl‐1,10‐phenanthroline) ligand in the solid state give [In(Tab) 2 (Dpphen) 2 ](PF 6 ) 3 ( 5 ) exclusively, regardless of the reaction stoichiometry. Compounds 2 , 4 and 5 associate with two fluorophore carboxyfluorescein (FAM) probe labelled DNA fragments (P‐DNAs) through electrostatic, π‐stacking and/or hydrogen‐bonding interactions, forming P‐DNA@ 2 , P‐DNA@ 4 and P‐DNA@ 5 hybrids that quench the fluorescence of FAM via a photo‐induced electron transfer process. These hybrids are further used as efficient and selective “turn‐on” fluorescent sensors for HIV‐1 ds‐DNA and Sudan Ebolavirus RNA sequences with detection limits in the pM–nM range.