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The Study of the Interaction between Doxorubicin and Single‐Stranded DNA
Author(s) -
Chen Xing,
Zhou Lu,
Wang Jine,
Jiang Guimei,
Cheng Hui,
Pei Renjun
Publication year - 2016
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201600473
Subject(s) - oligonucleotide , dna , chemistry , stacking , binding site , nucleotide , base pair , stereochemistry , doxorubicin , biochemistry , biology , genetics , gene , organic chemistry , chemotherapy
The binding properties of doxorubicin with single stranded DNA of various base compositions were determined by fluorescence and circular dischroism assays. The interaction of Dox with ssDNA comprising of single repeating deoxynucleotide was firstly investigated. Dox preferentially bound to the G residues among four bases. Then oligonucleotides covering potential GA, GT, GC or GG binding sites were designed to quantitatively analyze the preferential base composition at the binding site. Dox exhibited better effect on oligonucleotide with GC sites. To testify the binding preference to GC site, GC‐rich oligonucleotides containing two GC fragments with different array modes were employed. Dox was verified to preferentially bind to the GC site of single stranded DNA. The binding of Dox with GC site in single stranded DNA may involve in the cooperation interaction of multiple GC with one Dox molecule. It appears that Dox may prefer stacking interaction with the multiple GC nucleotide bases along the oligonucleotide backbone.