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Novel Reaction‐Based Fluorescence Probes for the Detection of Hydrogen Sulfide in Living Cells
Author(s) -
Zhao Zhensheng,
Cao Lixia,
Zhang Tao,
Hu Rui,
Wang Shuangqing,
Li Shayu,
Li Yi,
Yang Guoqiang
Publication year - 2016
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201600382
Subject(s) - bodipy , hydrogen sulfide , fluorescence , chemistry , tetrazine , selectivity , photochemistry , combinatorial chemistry , biophysics , biochemistry , biology , catalysis , organic chemistry , sulfur , quantum mechanics , physics
Hydrogen Sulfide (H 2 S), a kind of important gas signal molecules, serves as an important medium in physiological and pathological studies. It is also a hot spot in the research of chemistry and biology. We now report a new type of reaction‐based fluorescent probe for selective detecting H 2 S in living cells that adopts H 2 S reaction with tetrazine group (Tz) to dihydio‐tetrazine. The fluorescence probes, BODIPY−Tz‐I and BODIPY−Tz‐II (BODIPY: abbreviation for boron‐dipyrromethene), respond to H 2 S by a turn‐on fluorescence signal enhancement and show high selectivity of H 2 S over other biologically species. BODIPY−Tz‐I and BODIPY−Tz‐II can be used to detect H 2 S in both buffer solution and cells in vivo, which provide a novel and effective approach for H 2 S detection in biological systems.