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In situ Detection of a Single Bacterium in Complex Environment by Hyperspectral CARS Imaging
Author(s) -
Hong Weili,
Liao ChienSheng,
Zhao Hansen,
Younis Waleed,
Zhang Yinxin,
Seleem Mohamed N.,
Cheng JiXin
Publication year - 2016
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201600166
Subject(s) - hyperspectral imaging , in situ , bacteria , raman scattering , materials science , optics , environmental science , biomedical engineering , remote sensing , microbiology and biotechnology , chemistry , biology , raman spectroscopy , physics , medicine , geology , organic chemistry , genetics
Detection of bacteria is essential for clinical diagnosis of infectious disease and food safety purposes. However, it is still a challenging task to rapidly detect bacteria, especially in their natural environment. By fiber‐laser based hyperspectral coherent anti‐Stokes Raman scattering (CARS) imaging, we showed that bacteria can be detected in a single cell level using spectral focusing in the C−H stretching region, with a total recording time of 1‐2 minutes. With phase retrieval and multivariate curve resolution analysis, we further showed that a single bacterium can be detected in complex environment such as milk and urine, without need of culturing, labelling, or sample contact. Our work promises hyperspectral CARS imaging to be a mobile and inexpensive setup for in situ detection of bacteria and bacterial activity studies.