Premium
A Novel Enzymatic Strategy for the Synthesis of Substituted Tetrahydroisoquinolines
Author(s) -
Bonamore Alessandra,
Calisti Lorenzo,
Calcaterra Andrea,
Ismail Omar H.,
Gargano Maurizio,
D'Acquarica Ilaria,
Botta Bruno,
Boffi Alberto,
Macone Alberto
Publication year - 2016
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201600134
Subject(s) - chemistry , tetrahydroisoquinoline , stereoselectivity , yield (engineering) , aldehyde , diamine oxidase , enzyme , amine gas treating , pictet–spengler reaction , biocatalysis , substrate (aquarium) , enantioselective synthesis , enantiomeric excess , enantiomer , combinatorial chemistry , diamine , organic chemistry , stereochemistry , catalysis , reaction mechanism , materials science , oceanography , metallurgy , geology
A fully enzymatic asymmetric synthesis of substituted tetrahydroisoquinolines was achieved in two steps starting from dopamine and a set of amine substrates by coupling the activity of a plant diamine oxidase with the recombinant norcoclaurine synthase enzyme from Thalictrum flavum . In the first step, a variety of aliphatic and aromatic amines of general interest as pharmaceutical building blocks were transformed into the corresponding aldehydes by the broad specificity of diamine oxidase enzyme from Lathyrus cicera . In the second step, the stereoselectivity of the norcoclaurine synthase catalyzed reaction to yield (S)‐configured tetrahydroisoquinoline products was exploited by mixing the aldehyde obtained in the first step with dopamine, the committed substrate for the Pictet‐Spengler reaction. The reactions were carried out in aqueous buffer and the products thus obtained were extracted and characterized by chiral GC/MS. Enantiomeric excess > 99% were obtained for all substrates investigated. The method provided a set of novel tetrahydroisoquinolines with potentially interesting pharmacological profiles.