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XPS analysis of genomic DNA adsorbed on PEI‐modified surfaces
Author(s) -
Vanzetti Lia,
Pasquardini L.,
Potrich C.,
Vaghi V.,
Battista E.,
Causa F.,
Pederzolli C.
Publication year - 2016
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5932
Subject(s) - x ray photoelectron spectroscopy , polyethylenimine , cationic polymerization , adsorption , chemistry , dna , nucleic acid , polymer , combinatorial chemistry , chemical engineering , polymer chemistry , organic chemistry , biochemistry , gene , engineering , transfection
Polyethylenimine (PEI) is a cationic polymer able to interact with nucleic acids. Taking advantage of this property, PEI‐modified functional surfaces for the capture of DNA were developed and characterized with X‐ray photoelectron spectroscopy (XPS) during every step of the preparation. The XPS demonstrated the presence of PEI on the functional surfaces, while the density of PEI primary amino groups was quantified with a colorimetric test, resulting in 6 × 10 14 amino groups/cm 2 . Different amounts of DNA were adsorbed on the functional surfaces, and the interaction between DNA and PEI was monitored both via fluorescence and XPS. The functional surfaces were indeed able to capture DNA, opening the possibility of a low‐cost and fast strategy for the purification of DNA. Copyright © 2016 John Wiley & Sons, Ltd.