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Single cell lipidomics of SKBR‐3 breast cancer cells by using time‐of‐flight secondary‐ion mass spectrometry
Author(s) -
Ide Yoshimi,
Waki Michihiko,
Ishizaki Itsuko,
Nagata Yasuyuki,
Yamazaki Fumiyoshi,
Hayasaka Takahiro,
Masaki Noritaka,
Ikegami Koji,
Kondo Takeshi,
Shibata Kiyoshi,
Ogura Hiroyuki,
Sanada Noriaki,
Setou Mitsutoshi
Publication year - 2014
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5523
Subject(s) - lipidomics , breast cancer , chemistry , mass spectrometry , secondary ion mass spectrometry , ion , phosphocholine , cancer cell , cell , cancer , analytical chemistry (journal) , biochemistry , chromatography , phospholipid , membrane , medicine , organic chemistry , phosphatidylcholine
Breast cancer is the most common cancer among women worldwide. The molecular characterization of breast tumor cells by using single‐cell lipidomics remains relatively unexplored. Here, we introduce a time‐of‐flight secondary‐ion mass spectrometry (TOF‐SIMS) approach to visualize the lipids in individual breast cancer cells. The SKBR‐3 breast cancer cell line was cultured and dispersed into individual cells. After attachment to a substrate, the cells were rinsed with ammonium acetate and were analyzed using TOF‐SIMS. The instrument was operated with Bi 3 2+ as the primary ion. The distributions of ions, including positively charged phosphocholine, and negatively charged phosphates and fatty acids, were simultaneously visualized. These ions were distributed predominantly at the cell attachment sites. The signal intensities of fatty acid ions were determined from the mass spectra at the regions‐of‐interest. The results of fatty acid analyses on breast cancer cells were consistent with those of our previous study in which prominent expression of stearoyl‐CoA desaturase 1 in breast cancer cells was demonstrated. Static TOF‐SIMS was shown to be an effective method for determining the lipid molecular signature of the plasma membrane of individual breast cancer cells. Copyright © 2014 John Wiley & Sons, Ltd.