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ToF‐SIMS analysis of diadenosine triphosphate and didadenosine tetraphosphate using bismuth and argon cluster ion beams
Author(s) -
Shon Hyun Kyong,
Cho YoungLai,
Lim Choung Su,
Choi Joon Sig,
Chung Sang J.,
Lee Tae Geol
Publication year - 2014
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5514
Subject(s) - chemistry , hela , molecule , bismuth , cluster (spacecraft) , metabolite , extracellular , argon , ion , intracellular , mass spectrometry , ion beam , polyatomic ion , analytical chemistry (journal) , biochemistry , cell , chromatography , organic chemistry , computer science , programming language
In the past few decades, research has shown diadenosine polyphosphates (Ap n A) to be a family of intercellular and intracellular signaling molecules that play a key role in biological and pharmacological activities. ToF‐SIMS, recently shown to be a promising technique to analyze metabolites in biological samples due to its high sensitivity and specificity, was employed to analyze mixed diadenosine triphosphate (Ap 3 A) and diadenosine tetraphosphate (Ap 4 A) spiked in HeLa cell lysates by using bismuth and argon cluster ion beams. The molecular ion signals of Ap 3 A and Ap 4 A in the cell lysates were stronger when using an argon cluster ion beam than when using a bismuth cluster ion beam, although the detection limits of both molecules were the same. Using a correlation curve to indicate the molecular signal intensities and concentrations, the amount of Ap 3 A and Ap 4 A molecules in HeLa cell lysates could be quantified in the concentration range of 0.1 mM to 10 mM for Ap 3 A, and 5 mM and 20 mM for Ap 4 A. We believe that ToF‐SIMS analysis could be a powerful technique to detect important metabolite molecules such as Ap n A for biomedical applications, without the need for a separation process. Copyright © 2014 John Wiley & Sons, Ltd.