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Molecular imaging of biological tissue using gas cluster ions
Author(s) -
Tian Hua,
Wucher Andreas,
Winograd Nicholas
Publication year - 2014
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5509
Subject(s) - chemistry , ion , cluster (spacecraft) , mass spectrometry , mass spectrometry imaging , analytical chemistry (journal) , resolution (logic) , ionization , mass spectrum , molecule , maldi imaging , ion source , chemical ionization , signal (programming language) , chromatography , matrix assisted laser desorption/ionization , organic chemistry , computer science , programming language , adsorption , artificial intelligence , desorption
An Ar n + ( n = 1–6000) gas cluster ion source has been utilized to map the chemical distribution of lipids in a mouse brain tissue section. We also show that the signal from high mass species can be further enhanced by doping a small amount of CH 4 into the Ar cluster to enhance the ionization of several biologically important molecules. Coupled with secondary ion mass spectrometry instrumentation which utilizes a continuous Ar cluster ion projectile, maximum spatial resolution and maximum mass resolution can be achieved at the same time. With this arrangement, it is possible to achieve chemically resolved molecular ion images at the 4‐µm resolution level. The focused Ar n + /[Ar x (CH 4 ) y ] + beams (4–10 µm) have been applied to the study of untreated mouse brain tissue. A high signal level of molecular ions and salt adducts, mainly from various phosphocholine lipids, has been seen and directly used to map the chemical distribution. The signal intensity obtained using the pure Ar cluster source, the CH 4 ‐doped cluster source and C 60 is also presented. Copyright © 2014 John Wiley & Sons, Ltd.