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Characterization of IgG‐protein‐coated polymeric nanoparticles using complementary particle sizing techniques
Author(s) -
Minelli C.,
GarciaDiez R.,
Sikora A. E.,
Gollwitzer C.,
Krumrey M.,
Shard A. G.
Publication year - 2014
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5381
Subject(s) - dynamic light scattering , small angle x ray scattering , polystyrene , nanoparticle , particle (ecology) , scattering , materials science , sizing , particle size , chemical engineering , chemistry , shell (structure) , analytical chemistry (journal) , nanotechnology , chromatography , polymer , optics , composite material , organic chemistry , oceanography , physics , engineering , geology
When nanoparticles are introduced into biological media, they acquire a protein corona. The thickness of immunoglobulin G protein on 105 nm polystyrene particles has been determined by dynamic light scattering (DLS), differential centrifugal sedimentation (DCS) and small‐angle X‐ray scattering (SAXS). All three techniques measured an increase of the protein shell thickness with increasing concentration of the proteins during incubation with the nanoparticles. DLS measurements showed the highest increase, indicating a possible effect of particle agglomeration on the DLS results. DCS accuracy critically depends on the knowledge of the protein shell density but the data allows an estimation of the effective shell density when the thickness was independently determined. SAXS measurements revealed the complex core/shell structure of the bare polystyrene particles. © 2014 Crown Copyright. Surface and Interface Analysis © 2014 John Wiley & Sons Ltd.