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Detection of protein coatings on nanoparticles surfaces by ToF‐SIMS and advanced electron microscopy
Author(s) -
Neunzehn Jörg,
Draude Felix,
GollaSchindler Ute,
Arlinghaus Heinrich F.,
Wiesmann HansPeter
Publication year - 2013
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5287
Subject(s) - transmission electron microscopy , scanning electron microscope , secondary ion mass spectrometry , scanning transmission electron microscopy , nanoparticle , particle (ecology) , electron microscope , chemistry , nanotechnology , materials science , mass spectrometry , chemical engineering , analytical chemistry (journal) , crystallography , chromatography , optics , composite material , biology , physics , ecology , engineering
In this study, time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) and advanced electron microscopy (scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM)) were applied to detect and analyse different nano‐scaled protein coatings on gold nanoparticles (NP). The NP were coated with collagen type I and fibronectin as well as different combinations of these proteins. These two main proteins in human cell organization and tissue formation were identified with the aid of ToF‐SIMS by typical amino acid mass peak detection. In addition, the protein‐coated particles were investigated by TEM and SEM to get information about the protein structure, the protein layer thickness on the particle surfaces and the reaction of NP in different protein solutions. In this study, a differentiation of diverse protein induced particle agglomeration was proven. The investigations of this study were part of the Specific Targeted Research Project CellNanoTox (project no. NMP4‐CT‐2006‐032731) funded by the European Commission under the 6th EU Framework Programme for Research and Technological Development. Copyright © 2013 John Wiley & Sons, Ltd.