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Novel method to prepare biological samples using powerful magnets on TOF‐SIMS analysis
Author(s) -
Chang ChunChao,
Chen ChunNan,
Lin WeiJhih,
Lo TsuiYun,
Lei ShiouLing,
Mai FuDer
Publication year - 2013
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.5148
Subject(s) - secondary ion mass spectrometry , magnet , mass spectrometry , analytical chemistry (journal) , fracture (geology) , ion , sample preparation , chemistry , nanotechnology , materials science , chromatography , composite material , physics , organic chemistry , quantum mechanics
Imaging with time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) has been shown to be a powerful analytical tool for mapping the distribution of biologically relevant small molecules (<1000 Da) on a surface. To improve the success rate of sample preparation, a device was designed to cells freeze‐fracture by using powerful magnets. Quantum dots (QDs) were used to observe the changes in the cell after freeze‐fracture. Then, TOF‐SIMS was employed to analyze the ionic and molecular distribution in the cells associated with the disease. Copyright © 2012 John Wiley & Sons, Ltd.