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A systematic evaluation of cytospinning as a novel technique for the preparation of cells for ToF‐SIMS analysis
Author(s) -
Jackson E. R. J.,
Vickerman J. C.,
Lockyer N. P.
Publication year - 2011
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.3413
Subject(s) - hela , chemistry , suspension (topology) , analytical chemistry (journal) , materials science , nanotechnology , chromatography , cell , biochemistry , mathematics , homotopy , pure mathematics
In this paper we present a study focusing on the preparation of prostate cancer cell (PC‐3) nuclei and cervical cancer cells (Hela‐M) for ToF‐SIMS imaging. We evaluate cytospinning as a novel preparatory technique through comparisons with air drying, freeze drying, and freeze‐fracturing protocols and variations thereof. Using image contrast, assessing spectra for the loss, or gain of native and non‐native chemistry and relative organic and inorganic ion yields, the investigation provides a systematic evaluation of the features of each preparation technique. From this, cytospinning is shown to be a robust technique for the preparation of biological specimens from suspension. Using this technique, despite inconclusive SEM and SIMS images, it is likely the natural biochemistry and architecture was preserved, enabling accurate chemical localization. Copyright © 2010 John Wiley & Sons, Ltd.