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MeV‐energy probe SIMS imaging of major components in washed and fractured animal cells
Author(s) -
Yamada H.,
Nakata Y.,
Ninomiya S.,
Seki T.,
Aoki T.,
Tamura J.,
Matsuo J.
Publication year - 2011
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.3408
Subject(s) - secondary ion mass spectrometry , mass spectrometry , accelerator mass spectrometry , ion , analytical chemistry (journal) , chemistry , static secondary ion mass spectrometry , ion beam , ion beam analysis , atomic physics , physics , chromatography , organic chemistry
Molecular images of pretreated animal cells were measured by secondary ion mass spectrometry (SIMS) with MeV‐energy ion beam. SIMS analysis was performed for cell surfaces washed with buffers not only at high vacuum, but also at several tens Pa by using an orthogonal acceleration time of flight mass spectrometer (oaTOFMS) system. Moreover, molecular images of major components from washed and freeze fractured cells were obtained using MeV‐energy probe SIMS. Copyright © 2010 John Wiley & Sons, Ltd.