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Surface analytical characterization of carbohydrate microarrays
Author(s) -
Dietrich Paul M.,
Horlacher Tim,
Gross Thomas,
Wirth Thomas,
Castelli Riccardo,
Shard Alexander G.,
Alexander Morgan,
Seeberger Peter H.,
Unger Wolfgang E. S.
Publication year - 2010
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.3255
Subject(s) - x ray photoelectron spectroscopy , secondary ion mass spectrometry , biomolecule , chemistry , glycan , silane , mass spectrometry , dna microarray , surface modification , nanotechnology , analytical chemistry (journal) , materials science , chemical engineering , chromatography , organic chemistry , biochemistry , glycoprotein , gene expression , engineering , gene
Microarrays are a versatile platform for diagnostics and high‐throughput analysis. Carbohydrate microarrays are valuable tools to investigate interactions with other molecules since many glycans are involved in fundamental biological processes. A combined X‐ray photoelectron spectroscopy (XPS) and time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) surface analysis was used to investigate the basic steps in the production of carbohydrate microarrays. The preparation included coupling of a thiol‐terminated mannoside to maleimide‐functionalized glass surfaces derived from γ‐aminopropyl silane (GAPS) slides. XPS results clearly demonstrate successful chemical modification in each fabrication step, and ToF‐SIMS imaging revealed immobilized carbohydrates in the spotted regions of the final microarray. Copyright © 2010 John Wiley & Sons, Ltd.