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Bactericidal performance of photocatalytic titanium dioxide particle mixture under ultraviolet and fluorescent light: an in vitro study
Author(s) -
Koseki Hironobu,
Shiraishi Koutaro,
Tsurumoto Toshiyuki,
Asahara Tomohiko,
Baba Koumei,
Taoda Hiroshi,
Terasaki Nao,
Shindo Hiroyuki
Publication year - 2009
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.3087
Subject(s) - photocatalysis , titanium dioxide , oxidizing agent , particle size , irradiation , chemistry , ultraviolet , ultraviolet light , fluorescence , particle (ecology) , nuclear chemistry , photochemistry , materials science , organic chemistry , composite material , catalysis , physics , optoelectronics , quantum mechanics , nuclear physics , oceanography , geology
Abstract When ultraviolet (UV) light comes into contact with titanium dioxide (TiO 2 ), a variety of free radicals are released to provide a potent oxidizing power. Few reports are available, however, evaluating the bactericidal effects of TiO 2 particle under UV light and fluorescent light (FL) in the same line of research for clinical applications. In the present study, we set out to evaluate the in vitro photocatalytic bactericidal effects on Staphylococcus aureus , which is one of the most common pathogens of infectious disease, in an aqueous system of TiO 2 particles irradiated by UV and FL. A TiO 2 particle mixture containing 0.019 mg/ml of TiO 2 was prepared. A bacterial solution was added dropwise to the mixture, and the resulting product was irradiated by UV or FL light. The colony‐forming units were counted and the bacterial survival rate was calculated. Control samples maintained a relatively high bacterial survival rate. In the TiO 2 mixture group, however, the bacterial survival rate decreased steadily, reaching 9.4% after 60 min of exposure to UV light and 10.9% after 60 min of FL irradiation. Distributing the TiO 2 particles in a water mixture produces highly efficient light absorption and enables greater and more frequent adhesion with bacteria, allowing a high degree of photocatalytic antibacterial action. Although the quantity was inferior to UV, our TiO 2 particles were able to show effective bactericidal activity even under FL. The TiO 2 particle mixture is expected to prove effective in preventing postoperative infection. Copyright © 2009 John Wiley & Sons, Ltd.