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Use of differential charging to analyse microscopic specimens of Azolla leaves by XPS
Author(s) -
Genet Michel J.,
Jacques Christine,
Mozes Nava,
Hove Charles Van,
Lejeune André,
Rouxhet Paul G.
Publication year - 2002
Publication title -
surface and interface analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.52
H-Index - 90
eISSN - 1096-9918
pISSN - 0142-2421
DOI - 10.1002/sia.1427
Subject(s) - cuticle (hair) , cutin , fern , azolla , plant cuticle , botany , x ray photoelectron spectroscopy , epidermis (zoology) , chemistry , biophysics , biology , anatomy , chemical engineering , biochemistry , wax , engineering
Cavity cuticles that surround the bacterial symbionts residing in the leaf cavities of the aquatic fern Azolla and Azolla leaf cuticle on the epidermis outside the cavity (outer cuticle) were submitted to XPS analysis. Differential charging enabled the signals of the microscopic sample and the support signals to be differentiated. The cavity cuticle has a composition similar to cutin. In comparison with the outer cuticle, it is characterized by a lower concentration of aliphatic chains and a higher concentration of oxygen‐containing functions. This has implications in term of solute transport between the leaf and the bacteria. Copyright © 2002 John Wiley & Sons, Ltd.