Open AccessRapid induction of gliogenesis in OLIG2 and NKX2 .2‐expressing progenitors‐derived spheroids
Author(s) -
Yun Wonjin,
Kim In Yong,
Song Gwonhwa,
You Seungkwon
Publication year - 2020
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.19-0455
Subject(s) - olig2 , gliogenesis , neurosphere , biology , progenitor cell , organoid , neuroscience , induced pluripotent stem cell , microbiology and biotechnology , oligodendrocyte , stem cell , human brain , progenitor , neural stem cell , cellular differentiation , adult stem cell , central nervous system , embryonic stem cell , myelin , genetics , gene
Glial cells are crucial for the development of the central nervous system and the maintenance of chemical homeostasis. The process of gliogenesis has been well studied in the rodent brain, but it remains less well studied in the human brain. In addition, rodent glial cells differ from human counterparts in terms of morphologies, functions, and anatomical locations. Cerebral organoids (also referred to as spheroids) derived from human pluripotent stem cells (hPSCs) have been developed and are suitable cell‐based models for researching developmental and neurodegenerative diseases. The in vitro generation of glia, including astrocytes and oligodendrocytes, from such organoids represents a promising tool to model neuronal diseases. Here, we showed that three‐dimensional (3D) culture of OLIG2‐ and NKX2.2‐expressing neurospheres produced efficiently mature astrocytes and oligodendrocytes in terms of morphologies and expression pattern recapitulating native 3D environment. Our findings provide important insights for developmental research of the human brain and glial specification that may facilitate patient‐specific disease modeling.