Open AccessChondroitin Sulfate Glycosaminoglycan Scaffolds for Cell and Recombinant Protein‐Based Bone Regeneration
Author(s) -
Andrews Seth,
Cheng Albert,
Stevens Hazel,
Logun Meghan T.,
Webb Robin,
Jordan Erin,
Xia Boao,
Karumbaiah Lohitash,
Guldberg Robert E.,
Stice Steven
Publication year - 2019
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.18-0141
Subject(s) - bone morphogenetic protein 2 , chondroitin sulfate , bone morphogenetic protein , glycosaminoglycan , chemistry , mesenchymal stem cell , microbiology and biotechnology , bone morphogenetic protein 7 , regeneration (biology) , stem cell , bone healing , biomedical engineering , medicine , anatomy , biochemistry , biology , in vitro , gene
Bone morphogenetic protein 2 (BMP‐2)‐loaded collagen sponges remain the clinical standard for treatment of large bone defects when there is insufficient autograft, despite associated complications. Recent efforts to negate comorbidities have included biomaterials and gene therapy approaches to extend the duration of BMP‐2 release and activity. In this study, we compared the collagen sponge clinical standard to chondroitin sulfate glycosaminoglycan (CS‐GAG) scaffolds as a delivery vehicle for recombinant human BMP‐2 (rhBMP‐2) and rhBMP‐2 expression via human BMP‐2 gene inserted into mesenchymal stem cells (BMP‐2 MSC). We demonstrated extended release of rhBMP‐2 from CS‐GAG scaffolds compared to their collagen sponge counterparts, and further extended release from CS‐GAG gels seeded with BMP‐2 MSC. When used to treat a challenging critically sized femoral defect model in rats, both rhBMP‐2 and BMP‐2 MSC in CS‐GAG induced comparable bone formation to the rhBMP‐2 in collagen sponge, as measured by bone volume, strength, and stiffness. We conclude that CS‐GAG scaffolds are a promising delivery vehicle for controlling the release of rhBMP‐2 and to mediate the repair of critically sized segmental bone defects. Stem Cells Translational Medicine 2019;8:575–585